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Table 5 Effect of surfactants, commercial detergents and oxidizing agents on cellulase stability

From: Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07

Surfactants

Concentration (%)

Residual activity (%)

Control

 

100.0

Tween-40

0.1

119.7

1.0

89.6

Tween-60

0.1

124.9

1.0

93.0

Tween-80

0.1

116.4

1.0

87.0

Triton-X-100

0.1

105

1.0

92.5

SDS

0.1

130

1.0

95.7

Tide

0.1

90.7

1.0

75.5

Ariel

0.1

99.7

1.0

89.0

Surf Excel

0.1

94.3

1.0

80

Fena

0.1

89.4

1.0

68.4

Henko

0.1

88.2

1.0

55.9

Sodium

0.1 (%)

87.9

perborate

0.5 (%)

68.2

1.0 (%)

57.7

Sodium

0.1 (%)

127.2

hypochlorite

0.5 (%)

115.4

1.0 (%)

94.6

H2O2

0.1 (%)

118.7

1.0 (%)

96.9

  1. Enzyme was pre-incubated with different surfactants, commercial detergents and oxidizing agents at 65°C for 1 h and assayed as standard assay method. The enzyme activity without incubation with surfactants, commercial detergents and oxidizing agents was taken as 100%. Mean standard deviation for all the values is < ± 5.0%.
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BMC Biotechnology

ISSN: 1472-6750

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