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Table 4 Effect of different inhibitors on enzyme stability

From: Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07

Reagents Concentration Residual activity (%)
Control   100.0
β–mercaptoethanol 0.1 (%) 179.6
1.0 (%) 167.0
EDTA 5 mM 120.9
10 mM 110.5
Urea 5 mM 119.0
10 mM 114.0
IAA 5 mM 105.4
10 mM 70.0
p-CMB 5 mM 90.0
10 mM 50.0
DTT 5 mM 115.0
10 mM 134.0
  1. Enzyme was pre-incubated with different inhibitors at 65°C for 1 h and assayed as standard assay method. The enzyme activity without incubation with inhibitors was taken as 100%. Mean standard deviation for all the values is < ± 5.0%.
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BMC Biotechnology

ISSN: 1472-6750

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