Skip to main content

Table 3 Stability of cellulase in presence of various organic solvents

From: Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07

Organic solvents (30%) log P Residual activity (%)
1 h 24 h 48 h 72 h 96 h 120 h 144 h 168 h
Methanol −0.76 100.5 124.8 131.9 125.3 119.9 112.4 106.5 97.1
Iso-propanol −0.28 90.7 104.3 99.5 92.5 91.8 91.0 88.3 84.2
Ethanol −0.24 98 106.9 105.1 102.9 98.8 95.1 91.6 88.2
Benzene 2.13 99.7 112 120 109 96 89 85 79.9
Cyclohexane 3.3 90 118 133 123.9 112.9 109 93 89.9
Acetone −0.23 105.2 115.9 110.9 104.1 101 96 92 90.3
Butanol −0.80 120.7 160.3 145.2 130.4 122.6 112.3 107 100
Toluene 2.5 120.9 180.2 150.4 128.5 120.9 112.8 105.2 102.4
Iso-octane 2.9 120.5 170.3 135.6 130.2 123.2 120.4 115.6 100.2
Xylene 3.1 90 112 117 113 110 102 96 90
Hexane 3.6 128 143 124 128 113 114 104 100
n-decane 5.6 135.6 161.8 178.7 158.6 136.5 111.9 107 97.0
n-dodecane 6.0 141.6 170.7 242.5 168.4 179.8 130.5 113.0 100.0
  1. Enzyme was pre-incubated with different organic solvents at a concentration of 30% (v/v) at 65°C for different time period and assayed as standard assay method. The enzyme activity without incubation with organic solvent was taken as 100%. Mean standard deviation for all the values is < ± 5.0%.