BMC Biotechnology

Table 3 Stability of cellulase in presence of various organic solvents

From: Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07

Organic solvents (30%)	log P	Residual activity (%)
Organic solvents (30%)	log P	1 h	24 h	48 h	72 h	96 h	120 h	144 h	168 h
Methanol	−0.76	100.5	124.8	131.9	125.3	119.9	112.4	106.5	97.1
Iso-propanol	−0.28	90.7	104.3	99.5	92.5	91.8	91.0	88.3	84.2
Ethanol	−0.24	98	106.9	105.1	102.9	98.8	95.1	91.6	88.2
Benzene	2.13	99.7	112	120	109	96	89	85	79.9
Cyclohexane	3.3	90	118	133	123.9	112.9	109	93	89.9
Acetone	−0.23	105.2	115.9	110.9	104.1	101	96	92	90.3
Butanol	−0.80	120.7	160.3	145.2	130.4	122.6	112.3	107	100
Toluene	2.5	120.9	180.2	150.4	128.5	120.9	112.8	105.2	102.4
Iso-octane	2.9	120.5	170.3	135.6	130.2	123.2	120.4	115.6	100.2
Xylene	3.1	90	112	117	113	110	102	96	90
Hexane	3.6	128	143	124	128	113	114	104	100
n-decane	5.6	135.6	161.8	178.7	158.6	136.5	111.9	107	97.0
n-dodecane	6.0	141.6	170.7	242.5	168.4	179.8	130.5	113.0	100.0

Enzyme was pre-incubated with different organic solvents at a concentration of 30% (v/v) at 65°C for different time period and assayed as standard assay method. The enzyme activity without incubation with organic solvent was taken as 100%. Mean standard deviation for all the values is < ± 5.0%.

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