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Table 2 Effect of metal ions on enzyme activity and stability

From: Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07

Metal ions

Concentration (mM)

Residual activity (%)

Activity

Stability

Control

 

100.0

100.0

CaCl2

5

156.4

125.4

10

184.4

160.3

NiCl2

5

121.4

93.1

10

90.4

87.7

FeSO4

5

112.9

100.8

10

102

86.5

MgCl2

5

145.9

115.6

10

175.1

130.3

CuSO4

5

87.4

82.5

10

56.7

49.5

HgCl2

5

46

52

10

25

32

MnCl2

5

76.9

83.9

10

61.9

50.2

KCl

5

100.2

106.4

10

92.1

90.1

NaCl

5

127.8

101.4

10

141.4

125.3

ZnSO4

5

95

76

10

63

59

  1. Enzyme activity was determined at 65°C in the presence of metal ions in the reaction mixture directly and for stability enzyme was pre-incubated with different metal ions at 65°C for 1 h and assayed as standard assay method. The enzyme activity without incubation with metal ions was taken as 100%. Mean standard deviation for all the values is < ± 5.0%.
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BMC Biotechnology

ISSN: 1472-6750

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