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Table 2 Effect of metal ions on enzyme activity and stability

From: Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07

Metal ions Concentration (mM) Residual activity (%)
Activity Stability
Control   100.0 100.0
CaCl2 5 156.4 125.4
10 184.4 160.3
NiCl2 5 121.4 93.1
10 90.4 87.7
FeSO4 5 112.9 100.8
10 102 86.5
MgCl2 5 145.9 115.6
10 175.1 130.3
CuSO4 5 87.4 82.5
10 56.7 49.5
HgCl2 5 46 52
10 25 32
MnCl2 5 76.9 83.9
10 61.9 50.2
KCl 5 100.2 106.4
10 92.1 90.1
NaCl 5 127.8 101.4
10 141.4 125.3
ZnSO4 5 95 76
10 63 59
  1. Enzyme activity was determined at 65°C in the presence of metal ions in the reaction mixture directly and for stability enzyme was pre-incubated with different metal ions at 65°C for 1 h and assayed as standard assay method. The enzyme activity without incubation with metal ions was taken as 100%. Mean standard deviation for all the values is < ± 5.0%.