Figure 4

SDS-PAGE of different fractions of cellulase of Bacillus vallismortis RG-07 obtained during purification steps. Lane 1: Marker proteins; lane 2: Crude supernatant; lane 3: Concentrated enzyme after ammonium sulphate precipitation; lane 4: Purified enzyme obtained after anion-exchange chromatography; lane 5: Purified enzyme obtained after gel-filtration chromatography. Molecular weights were presented in the form of kDa.