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Table 2 Yields of cIFN-α expressed in optimized auto-induction system

From: Auto-induction expression of human consensus interferon-alpha in Escherichia coli

Sample

cIFN-α yield (g/L)

Total cell protein

1.24

Total cell lysate

1.08

Soluble cell lysate

0.76a (70.37%)

Solubilized inclusion bodies

0.217 (20.09%)

  1. aMajority of the expressed cIFN-α protein was in the cell lysate supernatant (about 70% of the yield in total cell lysate).