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Table 1 Reactivity of monoclonal anti-human IFN-α antibody against recombinant cIFN-α expressed in E. coli BL21 (DE3) cells by auto-induction

From: Auto-induction expression of human consensus interferon-alpha in Escherichia coli

Sample OD at 405 nm (mean ± SD)
Commercial IFN-α2a 0.934 ± 0.016
Recombinant cIFN-α (uninduced cells) 0.046 ± 0.008a
Recombinant cIFN-α (IPTG-induced cells) 0.447 ± 0.014b
Recombinant cIFN-α (auto-induced cells) 0.637 ± 0.022a,b
  1. aMonoclonal anti-human IFN-α antibody reacted significantly (P < 0.05) with expressed cIFN-α protein in auto-induced cells.
  2. bA significant difference (P < 0.05) in ELISA signals was found between recombinant cIFN-α in auto-induced cells and recombinant cIFN-α in IPTG-induced cells.