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Figure 2 | BMC Biotechnology

Figure 2

From: Auto-induction expression of human consensus interferon-alpha in Escherichia coli

Figure 2

SDS-PAGE analysis of auto-induction expression of cIFN-α. (A) Auto-induction expression of cIFN-α at 30°C for 12 and 24 h. Lane M: commercial IFN-α2a as molecular weight marker of 19,2 kDa size. Lane 1: total proteins from uninduced cells. Lane 2: total proteins from IPTG-induced cells. Lane 3: total proteins from auto-induced cells after 24 h incubation at 30°C. Lane 4: total proteins from auto-induced cells after overnight incubation at 30°C. (B) Auto-induction expression of soluble cIFN-α. Lane M: commercial IFN-α2a as molecular weight marker of 19,2 kDa size. Lanes 1–3, soluble cell lysates of auto-induced cells after 24 h incubation at 37°C, 30°C, and 25°C, respectively. The arrow heads pointed the position of cIFN-α.

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