Figure 1
From: Auto-induction expression of human consensus interferon-alpha in Escherichia coli
A 2% agarose gel showing the result of restriction digestion of recombinant pET-cIFNα. Recombinant pET-cIFNα was digested overnight at 37°C using XbaI and SacI enzymes. Lane 1 shows the pET-TOPO vector fragment (5.73 kb) and the cIFN-α band (534 bp, with 26 bp added to the actual size of the gene from the vector cloning site and 10 bp introduced by gene-specific primers in the gene to enable pET directional TOPO cloning). L pointed GeneRuler 100 bp Plus DNA Ladder.