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Figure 6 | BMC Biotechnology

Figure 6

From: Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coli

Figure 6

Lysis on a fibrin plate using urokinase and tPA. tPA-RGDS; HisTag-PSP-tPA-RGDS; urokinase standard (10 × 103 IU ml−1. positive control); BSA (bovine serum albumin standard, negative control). The fibrinolytic activity of tPA was measured and compared with urokinase (positive control) and bovine serum albumin (BSA; negative control). A, dot 1, 10 μg BSA; dot 2, 2.5 × 103 IU urokinase; dot 3, 5.0 × 103 IU urokinase (positive control); dot 4, 10 μg His-tag-PSP-tPA-RGDS; dot 5, 20 μg His-tag-PSP-tPA-RGDS; dot 6, 10 μg tPA-RGDS; dot 7, 20 ug tPA-RGDS. B, the diameters of the lysis dots on the fibrin plate from at four independent evaluations. The bars indicate standard error of the mean.

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