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Figure 4 | BMC Biotechnology

Figure 4

From: Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coli

Figure 4

Purification of recombinant tPA. A, SDS-PAGE analysis of tPA purity. Lane 1, protein markers; lane 2, lysate; lane 3, supernatant; lane 4, elution with 200 mM imidazole; lane 5, recombinant tPA purified using ion exchange chromatography; lane 6, PSP; lane 7, PSP enzyme reaction mixtures; lane 8, tPA purification using size-exclusion chromatography. B, Western blotting of the enzyme reaction products (lane 7 of Figure 4A) and the purified fraction (lane 8 of Figure 4A) using anti-tPA antibodies.

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BMC Biotechnology

ISSN: 1472-6750

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