Figure 2
Soluble expression of tPA in differentE. colistrains. After the auto-induction of tPA expression at 37°C for 24 h. The lysates from E coli, supernatant (soluble) fractions, flow-through proteins that did not bind to the column, and recombinant full-length tPA (His-tag-PSP-tPA-RGDS) were analyzed using SDS-PAGE and Coomassie blue staining. Lane 1, protein markers; lane 2, Origami 2 cell lysate; lane 3, Origami 2 supernatant; lane 4, flow-through; lane 5, elution with 200 mM imidazole from Ni2+-NTA; lane 6, BL21 lysate; lane 7, BL21 supernatant; lane 8, flow-through; lane 9, elution with 200 mM imidazole from Ni2+-NTA; lane 10, Rosetta™ 2 lysate; lane 11, Rosetta™ 2 supernatant; lane 12, flow-through; lane 13, elution with 200 mM imidazole from Ni2+-NTA.