Figure 6
From: Preparation of cell-permeable Cre recombinase by expressed protein ligation
Cellular imaging of the internalized ssCre-dR9-biotin. Human TE671 cells were treated with 80 μg/ml ssCre-dR9-biotin for 5 h (ssCre-dR9-biotin) or left untreated (Control). The cells were fixed and then sequentially incubated with avidin-FITC, anti-avidin-biotin antibody, and avidin-FITC. The fluorescence images were taken under a confocal laser scanning microscope.