Skip to main content
Figure 4 | BMC Biotechnology

Figure 4

From: Preparation of cell-permeable Cre recombinase by expressed protein ligation

Figure 4

Protein transduction of the semisynthetic Cre-R9 and Cre-dR9. (A) Cre-mediated recombination of the floxed STOP cassette leads to LacZ expression in human TE671 (LoxP-LacZ) cells. (B) The cells were exposed to varying doses of Cre, ssCre-R9, ssCre-dR9, and TAT-Cre in OPTI-MEM for 5 h. The cells were further incubated with the serum-containing media for a total of 48 h, and then the percentage of LacZ-expressing cells was determined by X-gal staining (n = 4; mean ± SD). The pCMV-Cre expression DNA plasmid was transfected using Lipofectamine.

Back to article page