Figure 1From: Preparation of cell-permeable Cre recombinase by expressed protein ligation General scheme for expressed protein ligation. In scheme 1, the Cre-thioester is isolated from the intein-His for further reaction with the thiol group of an N-terminal cysteine of the peptides. In scheme 2, the thiolysis by MESNA, the transthioesterification reaction, and the S-N acyl Shift proceed in one-pot.Back to article page