Figure 4

Two-dimensional heatmap illustrating VH–Vλ gene pairing frequencies in the unselected HAL9 library (uns.) and in the selected scFvs from the HAL9 library (s.). Frequency of all functional VH genes [46] and Vλ genes [47] are shown. Genes that are not confirmed to be functional, but were found in the library/were selected are marked in orange. 827 antibodies of the initial library and 704 selected scFvs were analyzed. The percentual amounts of each germline gene are given separetely for VH (rightmost columns) and Vλ (bottom row).