Figure 2
From: Generation and analysis of the improved human HAL9/10 antibody phage display libraries
Comparision of pHAL14 and pHAL30. (A) Comparision of the production of soluble antibodies. SN supernatant, PE periplasmatic extract, OS osmotic shock fraction, Eluate after IMAC purification, OD450nm-620nm optical density at 450Â nm (620Â nm reference), scFv antibody fragments analyzed: D1.3, HT186-D11 and TM43-E10, antigens: lysozyme, Muc-1 and OmpD. (B) Comparision of scFv-phage antigen binding. OD450nm-620nm optical density at 450Â nm (620Â nm reference), scFv antibody fragments D1.3, HT186-D11, SH313-B5 and TM43-E10. antigens: lysozyme, MUC1, CD30 and OmpD.