Figure 1
From: Generation and analysis of the improved human HAL9/10 antibody phage display libraries
Schematic drawing of pHAL14 and pHAL30. Abbreviations: lacZ promoter: promoter of the bacterial lac operon; RBS: ribosome binding site; pelB: signal peptide sequence of bacterial pectate lyase, mediating secretion into the periplasmic space; VH: variable fragment of the heavy chain; blue box: first six amino acids of CH1, part of the linker; LC: light chain; dark blue box: first 9 (kappa)/16 (lambda) aminoacids of CL; ochre: ochre stop codon; amber: amber stop codon; His-tag: 6xhistidine tag; terminator: sequence terminating transcription; Myc-tag: EQKLISEEDLN tag. The elements of the inserts are not drawn to scale.