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Table 3 Microbiological analysis of a HPW delivery system using developed methodology

From: A rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water

Sampling point

Sampling time

C T value (SD)

a Mean calculated cfu/100 ml (SD)

C T value (SD)

a Mean calculated cfu/100 ml (SD)

Above threshold EU/US guidelines action level of > 10 cfu/100 ml

  

S. maltophilia

S. maltophilia

Burkholderia

Burkholderia

A

1

29.02 (0.07)

7.7 × 101 (37)

27.14 (0.15)

7.4 × 102 (145)

Yes

A

2

29.25 (0.21)

6.5 × 101 (103)

27.81 (0.23)

6.2 × 102 (97)

Yes

A

3

29.40 (0.04)

5.8 × 101 (13)

28.47 (0.22)

4.1 × 102 (560)

Yes

B

1

27.90 (0.14)

1.7 × 102 (162)

27.64 (0.07)

6.9 × 102 (333)

Yes

B

2

28.30 (0.27)

1.3 × 102 (246)

27.16 (0.03)

9.4 × 102 (167)

Yes

B

3

28.10 (0.12)

1.5 × 102 (139)

26.83 (0.16)

1.2 × 103 (867)

Yes

  1. CT Threshold cycle.
  2. aMean calculated cfu/100 ml calculated by multiplex real time PCR.
  3. A Valve adjacent to Elix 35 HPW purification system.
  4. B Laboratory tap approximately 25 - 30 m from the Elix 35 HPW purification system.
  5. 1Before Pretreatment and Polishing Pack change.
  6. 2one day post Pretreatment and Polishing Pack change.
  7. 3one week post Pretreatment and Polishing Pack change.