Table 1 Oligonucleotide primers and probes used in this study

CIAC F

Forward composite primer for IAC generation

TGAGGAAGACTTATTGGCTGATACCCAACTTGGAATG

Chimeric PCR product

CIAC R

Reverse composite primer for IAC generation

CGGTACATTGTGGTCTTTAAGTCTTCACCAGAATAAAATTG

Chimeric PCR product

Ser F

Sequencing forward primer

GTGGAGCTGGGGGGAT

ssrA

Ser R

Sequencing forward primer

GGGGCTGATTCTGGATTCG

ssrA

Psd F1

Sequencing forward primer

GGATTTGAACCCCCGTCC

ssrA

Psd R2

Sequencing reverse primer

AGGATTCGACGCCGGT

ssrA

BF1

Sequencing forward primer

GAGGAAAGTCCGGACTCC

rnpB

BF2

Sequencing forward primer

GGCAGGGTGATGGCTAA

rnpB

BR2

Sequencing reverse primer

GATAAGCCGGATTCTGTGC

rnpB

Sten F

Sequencing forward primer

GGGGMYTACGGWTTCGAC

ssrA

Sten R

Sequencing forward primer

GGGARTCGAACCRSGTCC

ssrA

IAC F

IAC forward primer

TGAGGAAGACTTATTGGCTG

Chimeric PCR product

IAC R

IAC reverse primer

CGGTACATTGTGGTCTTTAAG

Chimeric PCR product

IAC P

IAC specific hydrolysis probe

TYE665-TCCTTAGATGGTACCAGTGCCA- IBRQSp

Chimeric PCR product

S. mar F

S. marcescens forward primer

GCACTACATGCTTAGTCCAAT

ssrA

S. mar R

S. marcescens reverse primer

CAGCTTAATACCCTGCTTAGAG

ssrA

S. mar P

S. marcescens specific hydrolysis probe

ROX-CACGCTACTGACAGACTAGCCT-BHQ2

ssrA

BF1

Burkholderia forward primer

GAGGAAAGTCCGGACTCC

rnpB

BR1

Burkholderia reverse primer

TCTTACCGCACCGTTTCA

rnpB

Burk P

Burkholderia genus specific hydrolysis probe

FAM-ACACGCGGAACAGGGCAA-BHQ1

rnpB

PF2

P. aeruginosa forward primer

GACAGTCGTTTCGGGTTTAC

ssrA

PR1

P. aeruginosa reverse primer

CGACGACAACTACGCTCT

ssrA

P. aer P

P. aeruginosa specific hydrolysis probe

HEX-GCATCCCCTAGCGACTGCT-BHQ1

ssrA

S. malt F

S. maltophilia forward primer

TACATGCTTAGCTCACCGT

ssrA

S. malt R

S. maltophilia reverse primer

CGAAACTGCTTGTGTCCAT

ssrA

S. malt P

S. maltophilia specific hydrolysis probe

CYAN500-CTGTTGTGTTCTAGTGCCGGAC-BHQ1

ssrA