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Figure 5 | BMC Biotechnology

Figure 5

From: The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri

Figure 5

Quantification and inducibility of luciferase activity in transformants. Transformants expressing the g-luc reporter gene under control of the nitA promoter and the parent wild-type strain were investigated for luciferase activity. The diagram shows the measured enzyme activity in untreated specimen (left column) and specimen induced by a switch from NH4 + to NO3 − (right column). The columns represent the mean of three independent experiments. The standard deviation is indicated. The factor of induction is given underneath the columns. The luciferase activity of each sample was normalized with its chlorophyll concentration. This luciferase activity assay was carried out more than 250 generations after transformation of the strains.

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