Figure 3

Analysis of paromomycin resistance in wild-type and transgenic V. carteri strains. For detailed analysis of resistance, identical quantities of V. carteri spheroids were exposed to increasing concentrations of paromomycin and incubated for six days. Numbers refer to the concentration of paromomycin [μg/ml] utilized. Natural color (left) and red-shifted, false-color images (right) are shown. (A) The wild-type V. carteri strain. (B) Transgenic V. carteri strain NitLuc-X171 that was co-transformed with pPmr3, the selectable marker plasmid, and plasmid pNitLucX, which carries the g-luc reporter gene driven by the nitA promoter. (C) Transgenic V. carteri strain NitLuc-A172 that was transformed only with plasmid pNitLucA, which carries both the selectable marker gene and the g-luc reporter gene driven by the nitA promoter.