Figure 4

Growth inhibitory effects of adenovirus encoding sTRAIL cells under the control of different Tet-On systems. (A) SW480 cells were treated with PBS (mock), Ad5-E1-eGFP, Ad5-E1-sTRAIL-E3-eGFP and Ad5-E1-KRAB-Bi-Tet-On-sTRAIL-E3-eGFP, respectively, at 250 MOI in the presence or absence of Dox. (B) U87 MG cells were treated similarly but with a less dose of virus at 100 MOI. Cell growth inhibition was analyzed by MTT assay three days post-transduction. Experiments were repeated four times; *P < 0.05, **P < 0.01.