Figure 3
From: Improving the large scale purification of the HIV microbicide, griffithsin
Effects of MgCl 2 (0, 0.001 M, 0.01 M, 0.1 M) on the protein content of GRFT extracts treated with bentonite. Coomassie stained SDS-PAGE was used to see gross changes in purity of the GRFT extracts in both a and b. The initial GRFT extract is represented in sample 1 and subsequent heat & centrifugation step at sample 2. The supernatant was treated with multiple MgCl2 concentrations (samples 5–12) in duplicate with the corresponding bentonite concentration 0 mg/mL bentonite (a) or 10 mg/mL bentonite (b). The resulting supernatants of overnight treatment with MgCl2 and/or bentonite are as follows; (3&4) 0 MgCl2 & 0 bentonite, (5&6) 0 MgCl2, (7&8) 0.001 M MgCl2, (9&10) 0.01 M MgCl2, (11&12) 0.1 M MgCl2. Samples 11 and 12 both show a dramatic reduction in coat protein after treatment with 0.1 M MgCl2 irrespective of the bentonite concentration. Table: Densitometry readings of SDS-PAGE represented in a and b including two additional bentonite concentrations not shown. The shaded area represents an extract condition achieving greater than 80% purity of GRFT.