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Table 1 Biochemical characterization of TrEGI expressed in different hosts

From: Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures

Expression host

Enzyme

T m (°C)

t 1/2 (hr)

 

50°C

60°C

65°C

70°C

S. cerevisiae

WT

57

-

-

-

3 (0.4)

S. cerevisiae

G230A/D113S/D115T

61

-

-

-

3.7 (0.5)

S. cerevisiae

WT + Glutaminyl Cyclase

65

-

-

-

-

N. crassa

WT

65

>5 days

70.6 (3.5)

4.0 (0.2)

3.3 (0.1)

N. crassa

G230A/D113S/D115T

68

>5 days

176 (16)

3.3 (0.3)

3.5 (0.4)

T. reesei

WT

65

>5 days

74 (2.5)

5.8 (0.6)

6.2 (0.4)

T. reesei

G230A/D113S/D115T

68

>5 days

161 (5)

6.2 (0.7)

6.4 (0.3)

  1. Tm is the apparent melting temperature for the enzyme, and t1/2 is half-life in hours. - = Not determined. Errors are reported in parentheses and represent one standard deviation for measurements. Apparent melting temperature data for Sc_TrEGI (with and without glutaminyl cyclase treatment) and Tr_TrEGI are shown in (Additional file 1: Figure S9). Apparent melting temperature data are not shown for other TrEGI enzymes. Half-life measurements for T. reesei- and N. crassa-expressed enzymes are shown in (Additional file 1: Figure S6 and Figure S7), respectively. Half-life measurements for S. cerevisiae-expressed TrEGI enzymes are not shown.