Figure 4From: Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures Temperature activity profile of wild-type T. reesei endoglucanase I (A-C) and G230A/D113S/D115T mutant (D-F) on Avicel (A,D), IL-Avicel (B,E), and MU-Cellobiose (C,F) expressed in S. cerevisiae , N. cra ssa, T. reesei , and S. cerevisiae followed by N-terminal cyclization of the wild-type TrEGI with gutaminyl cyclase. Error bars represent one standard deviation. Assays were carried out with purified TrEGI proteins (>95% pure based on SDS-PAGE analysis).Back to article page