Skip to main content

Table 2 Measurement of Copper (Cu) and Zinc (Zn) content of PschSOD by ICP-MS, Zincon and Bathocuproeine protocol

From: Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum

Proteins

ICP-MS

Zincon

Bathocuproeine

 

Cu

Zn

Cu

Zn

Cu

Zn

Bovine CuZn-SOD

1.9 ± 0.046

1.89 ± 0.05

1.92 ± 0.05

----

1.92 ± 0.06

----

PschSOD

1.84 ± 0.15

1.6 ± 0.12

1.82 ± 0.01

----

1.80 ± 0.08

----

  1. The values have been depicted as the quotient of concentration of metal ions to protein (dimer). The data presented here represents metal content from 3 independent protein preparations. There is no significant amount of Fe/Mn/ Ni or any other metal was found in the analysis.
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us