Skip to main content
Figure 6 | BMC Biotechnology

Figure 6

From: Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum

Figure 6

Sequence analysis and Homology modelling of PschSOD (A) Multiple sequence alignment was performed using ClustalW software (http://www.ebi.ac.uk/Tools/msa/clustalw2/). Accession no. of corresponding gene sequence are: Pisum sativum: emb|CAA39819.1|, Arabidopsis thaliana: gb|AAD10208.1|, Saccharomyces cerevisiae: ref|NP_012638.1|, Homo sapiens: pdb|1L3N|A and Escherichia coli: ref|WP_000875886.1|). Various conserved residue (His-44, His-46, His-61, and His-118) for Cu2+ and (His-61, His-69, His-78, and Asp-81) for Zn2+ has been marked with red and green arrow. Common Histidine (His-61) is also marked by black arrow while positively charged Arg-141 is marked by purple arrow. Chloroplast localization signal is present in SOD from plants only. (B) Tree was constructed using neighbor-joining method with 1000 bootstrap replicates. Monocot and dicot families are separated in two different clades. (C) Homology modelling of PschSOD was prepared by using default parameter at SWISS model workspace (http://swissmodel.expasy.org). The figure shows the dimer of modelled PschSOD with both Cu2+ and Zn2+ in active site. Active site is exposed outside in both monomer. A helix and beta sheets are also labelled. (D) All the co-ordinates of Cu2+ (Brown) and Zn2+ (Black) with His-61 being common in both cofactors show.

Back to article page