Figure 5From: Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum Longevity of PschSOD activity. Purified PschSOD was tested for its longevity both at room temperature and 4°C. Percentage residual activity was judged periodically with pyrogallol assay and further analyzed with native-PAGE and SOD specific staining after 180 days. The protein sample kept at −20°C was also judge for native activity and taken as control because its remains unaltered even after 180 days. Closed circle (●) represents the sample kept at room temperature while open circle (○) with solid line represents enzyme sample kept at 4°C.Back to article page