Figure 6
From: Synthesis of Fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreum
SDS-PAGE of the purified IslA4 inulosucrase form. The intracellular protein extracted from E.coli, was purified by affinity chromatography in Ni-nitroacetic acid (Ni-NTA) resin (Qiagen) and eluted with increasing amounts of imidazole. Afterwards, the enzyme was dialyzed against phosphate buffer and concentrated by ultrafiltration on an Amicon Ultra centrifugal Filter. 10,000 NMWL (Merck Millipore, Billerica, MA, USA).