Figure 3From: Synthesis of Fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreum Influence of enzyme concentration on the molecular weight distribution of inulin synthesized by IslA4 as observed by GPC. Reactions were carried out at three different enzyme concentrations (i.e., 1, 5 and 10 U mL−1 of IslA4) with 292 mM sucrose at 30°C in 50 mM phosphate (pH 6.0) containing 1 mM CaCl2, and were allowed to proceed until they reached a sucrose conversion of approximately 90%.Back to article page