Figure 2
From: Synthesis of Fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreum
Influence of substrate concentration on the IslA4 product profile, as observed by TLC. All of the reactions are carried out with 1 U mL−1 of IslA4 at 30°C in 50 mM phosphate (pH 6.0) containing 1 mM CaCl2, and were allowed to proceeded to a sucrose conversion of approximately 90%. Samples were taken at different time points. The numbers in the figures correspond to the following reaction times: 292 mM (0, 2, 4, 6, 8, 10, 12 and 24 h); 1170 mM (0, 3, 6, 9, 12, 25 and 34 h); and 2046 mM (0, 3, 6, 9, 12, 24, 27, 30, 34 and 48 h). The STD lane contained the following standards: G, glucose; F, fructose; S, sucrose; Kest, 1-kestose (GF2); Nyst, 1,1-nystose (GF3); F-nyst, fructofuranosyl-nystose; and Pol, polymer.