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Table 4 Dephosphorylation activity of mAP and commercial APs

From: A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments

Restriction enzyme

Dephosphorylation efficiency (%)

mAP

AAP

SAP

CIAP

HLAP

HindIII

100

99.8

99.5

99.9

99.8

PstI

91.1

87.4

98.0

98.2

95.2

SmaI

99.6

99.4

98.9

100

99.7

  1. mAP, metagenome-derived alkaline phosphatase; AAP, Antarctic alkaline phosphatase; SAP, shrimp alkaline phosphatase; CIAP, calf intestinal alkaline phosphatase; HLAP, APex heat-labile alkaline phosphatase. All results are the means of three experiments wherein variation was less than 10%.