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Table 3 Kinetic parameters of alkaline phosphatases

From: A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments

Enzyme

Temperature (°C)

K m (mM)

k cat (s −1 )

k cat /K m (s −1 M −1 )

Reference

ECAP

37

0.17

139

8.2 × 105

[13]

AAP

30

0.50

236

4.7 × 105

[27]

SAP

37

0.69

7,176

1.0 × 107

[28]

CIAP

37

0.40

43

1.0 × 105

[29]

SMAP

37

0.085

2.3

2.7 × 104

[22]

mAP

37

0.18

952

5.3 × 106

This study

  1. ECAP, E. coli alkaline phosphatase; AAP, Antarctic alkaline phosphatase; SAP, shrimp alkaline phosphatase; CIAP, calf intestinal alkaline phosphatase; SMAP, Sinorhizobium meliloti alkaline phosphatase; mAP, metagenome-derived alkaline phosphatase.
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BMC Biotechnology

ISSN: 1472-6750

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