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Table 2 Effects of metal ions on the activity of mAP

From: A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments

Metal ions (1 mM)

% AP activity restored*

None

0.11

Ca2+

45.6

Co2+

27.3

Zn2+

12.8

Ni2+

1.66

Mn2+

1.44

Mg2+

0.81

Cu2+

0.24

  1. *Results are expressed as the percentage of activity restored when compared with the enzyme that was not dialyzed against 5 mM EDTA (see Methods). All results are the means of three experiments wherein variation was less than 10%.