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Table 1 Substrate specificity of purified mAP

From: A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments

Substrates (1 mM)

P i released relative to that from p NPP*

pNPP

100

bis-pNPP

ND

AMP

39

ADP

76

ATP

25

dATP

44

dCTP

38

dGTP

39

dTTP

37

D-Glucose 1-phosphate

0.2

O-Phosphoethanolamine

3

Phosphoenolpyruvate

18

Phytic acid

ND

Paraoxaon

ND

Methyl parathion

ND

  1. *The P i released from pNPP was set at 100%. ND: not detected. All results are the means of three experiments wherein variation was less than 15%.