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Table 1 The hydrolysis of various substrates catalyzed by RpGla and RpAmy

From: Cloning of a novel thermostable glucoamylase from thermophilic fungus Rhizomucor pusillus and high-level co-expression with α-amylase in Pichia pastoris

Substrate Relatively activity (%)
  RpGla RpAmy Co-expression a
Soluble starch 55.8 ± 3.1 54.2 ± 2.7 100
maize starch 28.6 ± 0.8 34.9 ± 1.7 61.5 ± 2.8
potato starch 30.6 ± 1.8 28.8 ± 1.5 51.6 ± 2.4
amylopectin 66.4 ± 2.0 29.9 ± 0.7 79.2 ± 2.1
amylose 6.0 ± 0.2 5.2 ± 0.2 10.1 ± 0.4
glycogen 5.5 ± 0.2 5.8 ± 0.3 12.0 ± 0.5
  1. aenzyme preparation produced by KM71/9KGla-ZαAmy. Substrates (0.05 g) in 0.1 M citric acid-sodium citrate buffer (pH 5.0, 5 ml) was mixed with 0.5 ml appropriately diluted enzyme. The reaction was carried out at 60°C for 10 min, and terminated by adding 1 ml of 3, 5-dinitrosalicylic acid and boiled for 5 minutes.