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Table 1 The hydrolysis of various substrates catalyzed by RpGla and RpAmy

From: Cloning of a novel thermostable glucoamylase from thermophilic fungus Rhizomucor pusillus and high-level co-expression with α-amylase in Pichia pastoris

Substrate

Relatively activity (%)

 

RpGla

RpAmy

Co-expression a

Soluble starch

55.8 ± 3.1

54.2 ± 2.7

100

maize starch

28.6 ± 0.8

34.9 ± 1.7

61.5 ± 2.8

potato starch

30.6 ± 1.8

28.8 ± 1.5

51.6 ± 2.4

amylopectin

66.4 ± 2.0

29.9 ± 0.7

79.2 ± 2.1

amylose

6.0 ± 0.2

5.2 ± 0.2

10.1 ± 0.4

glycogen

5.5 ± 0.2

5.8 ± 0.3

12.0 ± 0.5

  1. aenzyme preparation produced by KM71/9KGla-ZαAmy. Substrates (0.05 g) in 0.1 M citric acid-sodium citrate buffer (pH 5.0, 5 ml) was mixed with 0.5 ml appropriately diluted enzyme. The reaction was carried out at 60°C for 10 min, and terminated by adding 1 ml of 3, 5-dinitrosalicylic acid and boiled for 5 minutes.
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BMC Biotechnology

ISSN: 1472-6750

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