|
Substrate
|
Relatively activity (%)
|
|---|
| |
RpGla
|
RpAmy
|
Co-expression a
|
|---|
|
Soluble starch
|
55.8 ± 3.1
|
54.2 ± 2.7
|
100
|
|
maize starch
|
28.6 ± 0.8
|
34.9 ± 1.7
|
61.5 ± 2.8
|
|
potato starch
|
30.6 ± 1.8
|
28.8 ± 1.5
|
51.6 ± 2.4
|
|
amylopectin
|
66.4 ± 2.0
|
29.9 ± 0.7
|
79.2 ± 2.1
|
|
amylose
|
6.0 ± 0.2
|
5.2 ± 0.2
|
10.1 ± 0.4
|
|
glycogen
|
5.5 ± 0.2
|
5.8 ± 0.3
|
12.0 ± 0.5
|
- aenzyme preparation produced by KM71/9KGla-ZαAmy. Substrates (0.05 g) in 0.1 M citric acid-sodium citrate buffer (pH 5.0, 5 ml) was mixed with 0.5 ml appropriately diluted enzyme. The reaction was carried out at 60°C for 10 min, and terminated by adding 1 ml of 3, 5-dinitrosalicylic acid and boiled for 5 minutes.
