Figure 2From: Cloning of a novel thermostable glucoamylase from thermophilic fungus Rhizomucor pusillus and high-level co-expression with α-amylase in Pichia pastorisGlucoamylase and α-amylase production of recombinant P. pastoris . (a) Time course of saccharogenic activity of KM71/9KGla (■) and KM71/9KGla-ZαAmy (●); (b) Time course of dextrinogenic activity of KM71/ZαAmy (■) and KM71/9KGla-ZαAmy (●). The induction were performed in 500 ml shake flask with 50 ml BMMY media incubating in 30°C, 230 rpm. The induction period was 5 days with the addition of 0.5% (v/v) methanol per day.Back to article page