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Figure 1 | BMC Biotechnology

Figure 1

From: Cloning of a novel thermostable glucoamylase from thermophilic fungus Rhizomucor pusillus and high-level co-expression with α-amylase in Pichia pastoris

Figure 1

SDS-PAGE analysis of recombinant RpGla and RpAmy expressed in recombinants P. pastoris KM71. (a): Lane 1. 10 μl of supernatant of recombinant KM71/9KGla-ZαAmy; Lane 2. 10 μl of supernatant of recombinant KM71/9KGla; Lane 3. 30 μl of supernant of recombinant KM71/ZαAmy; Lane 4. 10 μl of supernatant of recombinant transformed with empty vector pPIC9K; Lane 5. 10 μl of supernatant of recombinant transformed with empty vector pPICZα; Lane M. Molecular weight marker of proteins. (b): Lane 1, 10 μl of recombinant RpGla obtained by partial purification; (c): Lane 1, 10 μl of recombinant RpAmy obtained by partial purification. (d) Line 1, 10 μl of deglycosylated recombinant RpGla; Lane 2, 10 μl of recombinant RpGla; Line 3, 10 μl of deglycosylated recombinant RpAmy; Line 4, 10 μl of recombinant RpAmy.

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BMC Biotechnology

ISSN: 1472-6750

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