TY - JOUR AU - He, Zhenggui AU - Zhang, Lujia AU - Mao, Youzhi AU - Gu, Jingchao AU - Pan, Qi AU - Zhou, Sixing AU - Gao, Bei AU - Wei, Dongzhi PY - 2014 DA - 2014/12/24 TI - Cloning of a novel thermostable glucoamylase from thermophilic fungus Rhizomucor pusillus and high-level co-expression with α-amylase in Pichia pastoris JO - BMC Biotechnology SP - 114 VL - 14 IS - 1 AB - Fungal amylase, mainly constitute of fungal α-amylase and glucoamylase, are utilized in a broad range of industries, such as starch hydrolysis, food and brewing. Although various amylases have been found in fungi, the amylases from Aspergillus dominate the commercial application. One of main problems exist with regard to these commercial use of amylases is relatively low thermal and acid stability. In order to maximize the efficiency of starch process, developing fungal amylases with increased thermostability and acid stability has been attracting researchers’ interest continually. Besides, synergetic action of glucoamylase and α-amylase could facilitate the degradation of starch. And co-expressing glucoamylase with α-amylase in one host could avoid the need to ferment repeatedly and improves cost-effectiveness of the process. SN - 1472-6750 UR - https://doi.org/10.1186/s12896-014-0114-8 DO - 10.1186/s12896-014-0114-8 ID - He2014 ER -