HIF- 1α IHC for kidney (×200) and Percentage of HIF-1α positive cells in the AV loop. The HIF-1α IHC for kidneys taken under ×200 magnification. In control group (A) and DMOG-1 group (B) the kidneys were negative. In DMOG-2 group (C) HIF-1α positive cells were intensive brown in nuclei (pointed by arrows). (D) The slides from the central part of the Loop was cut and stained for HIF-1α IHC. In each slide 8 ROIs were taken from the up, down, left and right direction to the central artery or vein under x400 magnification. The brown nuclei cells recognized as HIF-1α positive cells and the blue nuclei cells recognized as HIF-1α negative cells, so the percentage of percentage of HIF-1α positive cells could be calculated. The DMOG-2 group that received DMOG i.p. injection in 15mg/KG at day 8, day10 and day12 had a higher HIF-1α positive rate than the control group. (48% versus 10% , *p < 0.05).