Table 6 Primers for SDV rHA proteins of H3 A/Perth and 5Cys SDV rHA of H3 A/Texas
Substitutions (SDV) | Primers (5′ to 3′) | |
|---|---|---|
H3 A/Perth | ||
C524S, C528A (Control SDV) | forward | CCTTTGCCATATCATcTTTTTTGCTTgcTGTTGCTTTGTTGGGG |
reverse | CCCCAACAAAGCAACAgcAAGCAAAAAAgATGATATGGCAAAGG | |
C539A, C546A,C549A (3Cys) | forward | GGGGTTCATCATGTGGGCCgcCCAAAAAGGCAACATTAGGgcCAACATTgcCATTTAAGTAAGTACCG |
reverse | CGGTACTTACTTAAATGgcAATGTTGgcCCTAATGTTGCCTTTTTGGgcGGCCCACATGATGAACCCC | |
C539A, C546A, C549A, C524A, C528A (5Cys)* | forward | CCTTTGCCATATCAgcTTTTTTGCTTgcTGTTGCTTTGTTGGGG |
reverse | CCCCAACAAAGCAACAgcAAGCAAAAAAgcTGATATGGCAAAGG | |
C64S,C76S (2Cys) | forward | CCTCATCAGATCCTTGATGGAAAAAACTctACACTAATAGATGCTCTATTGGGAGACCCTCAGTcTGATGGCTTCCAAAATAAGAAATGGG |
reverse | CCCATTTCTTATTTTGGAAGCCATCAgACTGAGGGTCTCCCAATAGAGCATCTATTAGTGTagAGTTTTTTCCATCAAGGATCTGATGAGG | |
H3 A/Texas | ||
C539A, C546A, C549A (3Cys) | forward | CATCATGTGGGCCgcCCAAAAGGGCAACATTAGGgcCAACATTgcCATTTGATAAGTAA |
reverse | TTACTTATCAAATGgcAATGTTGgcCCTAATGTTGCCCTTTTGGgcGGCCCACATGATG | |
C524A, C528A, C539A, C546A, C549A (5Cys)* | forward | TCCTTTGCCATATCAGcTTTTTTGCTTgcTGTTGCTTTGTTGGGG |
reverse | CCCCAACAAAgcAACAGCAAGCAAAAAAgCTGATATGGCAAAGGA | |