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Table 6 Primers for SDV rHA proteins of H3 A/Perth and 5Cys SDV rHA of H3 A/Texas

From: Modifications of cysteine residues in the transmembrane and cytoplasmic domains of a recombinant hemagglutinin protein prevent cross-linked multimer formation and potency loss

Substitutions (SDV) Primers (5′ to 3′)
H3 A/Perth
C524S, C528A (Control SDV) forward CCTTTGCCATATCATcTTTTTTGCTTgcTGTTGCTTTGTTGGGG
reverse CCCCAACAAAGCAACAgcAAGCAAAAAAgATGATATGGCAAAGG
C539A, C546A,C549A (3Cys) forward GGGGTTCATCATGTGGGCCgcCCAAAAAGGCAACATTAGGgcCAACATTgcCATTTAAGTAAGTACCG
reverse CGGTACTTACTTAAATGgcAATGTTGgcCCTAATGTTGCCTTTTTGGgcGGCCCACATGATGAACCCC
C539A, C546A, C549A, C524A, C528A (5Cys)* forward CCTTTGCCATATCAgcTTTTTTGCTTgcTGTTGCTTTGTTGGGG
reverse CCCCAACAAAGCAACAgcAAGCAAAAAAgcTGATATGGCAAAGG
C64S,C76S (2Cys) forward CCTCATCAGATCCTTGATGGAAAAAACTctACACTAATAGATGCTCTATTGGGAGACCCTCAGTcTGATGGCTTCCAAAATAAGAAATGGG
reverse CCCATTTCTTATTTTGGAAGCCATCAgACTGAGGGTCTCCCAATAGAGCATCTATTAGTGTagAGTTTTTTCCATCAAGGATCTGATGAGG
H3 A/Texas
C539A, C546A, C549A (3Cys) forward CATCATGTGGGCCgcCCAAAAGGGCAACATTAGGgcCAACATTgcCATTTGATAAGTAA
reverse TTACTTATCAAATGgcAATGTTGgcCCTAATGTTGCCCTTTTGGgcGGCCCACATGATG
C524A, C528A, C539A, C546A, C549A (5Cys)* forward TCCTTTGCCATATCAGcTTTTTTGCTTgcTGTTGCTTTGTTGGGG
reverse CCCCAACAAAgcAACAGCAAGCAAAAAAgCTGATATGGCAAAGGA
  1. *DNA for the corresponding triple mutation (3Cys) was used as the template for the PCR reaction.
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BMC Biotechnology

ISSN: 1472-6750

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