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Figure 8 | BMC Biotechnology

Figure 8

From: Modifications of cysteine residues in the transmembrane and cytoplasmic domains of a recombinant hemagglutinin protein prevent cross-linked multimer formation and potency loss

Figure 8

A-C Experimental data for H3 A/Texas 5Cys SDV and wild-type rHA stored at 5°C and 25°C. Potency by SRID, cross-linking by SDS-PAGE, and hydrophobicity by RP-HPLC were monitored monthly for 12 months. A. Shown are the potency results obtained at each time point for the rHA proteins stored at 5°C (top graph) and 25°C (bottom graph). For each rHA, the potency results are plotted relative to the day 0 value. B. Shown are the non-reducing and reducing SDS-PAGE results obtained on day 0 and after 12 months for the sets of wild-type and 5Cys SDV rHA proteins stored at 5°C and 25°C. C. Shown are the RP-HPLC profiles obtained on day 0 and 12 months for the sets of wild-type and 5Cys SDV rHA proteins stored at 5°C and 25°C.

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