A-4C SEC-HPLC elution profiles, SEC-UPLC elution profiles, and thermal denaturation curves using DSF for the rHA proteins. A. The SEC-HPLC elution profiles for the wild-type and SDV rHA proteins overlay. The retention times and extrapolated MWs are similar for the rHA proteins. The # of trimers per rosette was estimated using the extrapolated MW and a trimer MW of ~210 kDa. B. The SEC-UPLC elution profiles before (solid lines) and after (dotted lines) Zwittergent 3–14 pre-treatment are shown in for the wild-type (left) and 5Cys SDV rHA (right). C. The melting temperature (Tm) was determined by DSF, and the second derivative thermal denaturation curves are shown for each rHA.