Figure 5
From: Protein surface charge of trypsinogen changes its activation pattern
Autoactivation of trypsinogen at pH 8 and different salt concentrations. Trypsinogen autoactivation was measured in 100 mmol/L Tris-HCl pH 8, 1 mmol/L CaCl2 and 100 mmol/L NaCl at 37°C with 2 μmol/L trypsinogen and 10 nmol/L trypsin as an initial starting concentration. Trypsin activity was determined with Cbz-GPR-pNA as substrate.