Figure 4From: Protein surface charge of trypsinogen changes its activation patternSDS-PAGE analysis of autoactivation of human trypsinogen. The samples were analyzed on a 15% reducing SDS-PAGE gel using silver staining. Reactions were terminated by adding SDS-PAGE sample buffer followed by an immediate denaturation step for 5 min at 95°C (Tg = trypsinogen; Tr = trypsin).Back to article page