Table 1 Amount of biomolecules used in cultivation and quantification of adsorbed HA and TGF-β3 within gelatin scaffolds at day 21 of cultivation
Name of experimental group | Amount of biomolecules used in curtivation | Amount of biomolecules remained in scaffolds at day 21 | ||||
|---|---|---|---|---|---|---|
TGF-β3 (ng) | HA (μg) | TGF-β3 (ng) | HA (μg) | |||
PBS control | - | - | - | - | ||
Soluble control (X 6 times of culture media exchange) | 60.0 | 420.0 | - | - | ||
HA adsorbed scaffold | - | 70.0 | - | 67.24 ± 0.37 | (96.06 ± 0.53%) | |
TGFβ3 adsorbed scaffold | 10.0 | - | 9.48 ± 0.098 | (94.8 ± 0.98%) | - | |
HA + TGFβ3 adsorbed scaffold | 10.0 | 70.0 | 9.71 ± 0.11 | (97.1 ± 1.10%) | 66.12 ± 0.23 | (94.46 ± 0.32%) |