Glycosaminoglycans concentration in chondrocyte-seeded adsorbed scaffold culture media. Human articular chondrocytes were cultured for 21 days, and Glycosaminoglycans, which were released into the culture media, were measured at 3-day intervals (A). HA accumulation in the culture media (B). s-GAG released into the culture media at 3-day intervals (C). s-GAG accumulation in the culture media (D) were measured (n = 3). *, # p-values are < 0.05; **, ## p-values are < 0.01; * is a significant amount compared to the PBS control; # is a significant amount compared to the soluble control.