Saccharification of rice straw by cellulase from a local Trichoderma harzianum SNRS3 for biobutanol production

BMC Biotechnology

Table 3 Reducing sugar production (g/L) from rice straw using various concentrations of crude cellulase from T. harzianum SNRS3

	Enzyme Concentration (U/g substrate)
Time (h)	*1	*2	*3	*4	*5
0	0.00^Aa	0.00^Aa	0.00^Aa	0.00^Aa	0.00^Aa
24	4.31^Ba	11.19^Bb	15.05^Bc	21.14^Bd	21.34^Bd
48	4.98^Ca	12.78^Cb	17.40^Cc	26.93^Cd	26.20^Cd
72	5.82^Da	14.57^Db	20.14^Dc	29.87^Dd	30.55^Dd
96	5.84^Da	14.77^Db	20.31^Dc	30.47^Dd	30.87^Dd

Note: *1: FPase 6.25 U/g, CMCase 111.31 U/g, β-glucosidase 173.71 U/g.
*2: FPase 31.25 U/g, CMCase 566.55 U/g, β-glucosidase 868.55 U/g.
*3: FPase 62.5 U/g, CMCase 1113.1 U/g, β-glucosidase 1737.1 U/g.
*4: FPase 93.75 U/g, CMCase 1669.65 U/g, β-glucosidase 2605.65 U/g.
*5: FPase 125 U/g, CMCase 2226.2 U/g, β-glucosidase 3474.2 U/g.
Means expressed with different superscript capital letters within the same column was significantly different at p < 0.05.
Means expressed with different superscript small letters within the same row was significantly different at p < 0.05.