Table 3 Specificity of glycan binding proteins for hGGT1 from kidney, liver and Pp -hGGT1
Signal 2X higher than background | ||||
|---|---|---|---|---|
Detection | Specificity | Kidney | Liver | Pp-hGGT1 |
Anti-GGT1 | hGGT1 | Yes | Yes | Yes |
Microvirin (MVN) | Mannose | No | No | Yes |
Concanavalin A (ConA) | terminal α-Man | No | No | Yes |
Galanthus nivalis (GNL) | Man(terminal Manα(1-3)Man) | No | No | Yes |
Euonymus europaeus (EEL) | Galα(1-3)Gal | Yes | No | Yes |
Erythrina cristagalli (ECL) | Terminal Lac/LacNAc | Yes | No | Yes |
Griffonia simplicifolia (GSL-1) | α-GalNAc, GalNAc α-Thr/Ser(T) and α-Gal | Yes | No | Yes |
Datura stramonium (DSL) | (GlcNAc)n, polyLacNAc and LacNAc | Yes | Yes | No |
Phaseolus vulgaris Erythroagglutinin(Pha-E) | Galb(1-4)GlcNAcβ(1-2)Man | Yes | No | No |